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PRSM

construct_qc

Active

Tool of com.seqbench/workbench

declared in 1.1.0

Lint a coding DNA sequence for premature stops, internal RBS/polyA motifs, unwanted restriction sites, GC extremes and repeats.

Parameters schema

{
  "type": "object",
  "required": [
    "sequence"
  ],
  "properties": {
    "gcLow": {
      "type": "number",
      "default": 30,
      "description": "GC% below this flags an AT-rich window."
    },
    "gcHigh": {
      "type": "number",
      "default": 70,
      "description": "GC% above this flags a GC-rich window."
    },
    "gcWindow": {
      "type": "integer",
      "default": 50,
      "description": "Sliding-window size (nt) for GC-extreme scanning."
    },
    "sequence": {
      "type": "string",
      "description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
    },
    "frameStart": {
      "type": "integer",
      "default": 1,
      "description": "1-based nucleotide where the reading frame begins."
    },
    "avoidEnzymes": {
      "type": "array",
      "items": {
        "type": "string"
      },
      "description": "Enzyme names whose internal sites should be flagged as errors."
    },
    "homopolymerMin": {
      "type": "integer",
      "default": 8,
      "description": "Minimum run length to flag a homopolymer."
    },
    "crypticOrfMinAa": {
      "type": "integer",
      "default": 20,
      "description": "Minimum peptide length (aa) for a hidden alternate-frame ORF to be flagged."
    }
  },
  "additionalProperties": false
}

What this tool wraps· 0 endpoints

min confidence0.700.50

No endpoints wrapped at confidence ≥ 0.70.

Parent server

com.seqbench/workbench

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