construct_qc
ActiveTool of com.seqbench/workbench
declared in 1.1.0
Lint a coding DNA sequence for premature stops, internal RBS/polyA motifs, unwanted restriction sites, GC extremes and repeats.
Parameters schema
{
"type": "object",
"required": [
"sequence"
],
"properties": {
"gcLow": {
"type": "number",
"default": 30,
"description": "GC% below this flags an AT-rich window."
},
"gcHigh": {
"type": "number",
"default": 70,
"description": "GC% above this flags a GC-rich window."
},
"gcWindow": {
"type": "integer",
"default": 50,
"description": "Sliding-window size (nt) for GC-extreme scanning."
},
"sequence": {
"type": "string",
"description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
},
"frameStart": {
"type": "integer",
"default": 1,
"description": "1-based nucleotide where the reading frame begins."
},
"avoidEnzymes": {
"type": "array",
"items": {
"type": "string"
},
"description": "Enzyme names whose internal sites should be flagged as errors."
},
"homopolymerMin": {
"type": "integer",
"default": 8,
"description": "Minimum run length to flag a homopolymer."
},
"crypticOrfMinAa": {
"type": "integer",
"default": 20,
"description": "Minimum peptide length (aa) for a hidden alternate-frame ORF to be flagged."
}
},
"additionalProperties": false
}No endpoints wrapped at confidence ≥ 0.70.
Parent server
com.seqbench/workbench
1/7 registries