prime_editing_twin_design
ActiveTool of com.seqbench/workbench
declared in 1.1.0
Design a twinPE pegRNA pair (Anzalone et al. 2022) for a replacement too large for a single pegRNA's RTT: a left pegRNA nicks the + strand at/before the replacement window and a right pegRNA nicks the - strand at/after it, each synthesizing a new 3' flap; both flaps are truncated at a shared overlap in the middle of the new sequence so they anneal and resolve the edit without an HDR donor. Off-target activity is not evaluated (no in-browser reference genome).
Parameters schema
{
"type": "object",
"required": [
"target",
"replaceStart",
"replaceEnd",
"newSequence"
],
"properties": {
"target": {
"type": "string",
"description": "Forward-strand target DNA (raw or FASTA), with flanking sequence on both sides of the replacement window."
},
"pbsLength": {
"type": "integer",
"description": "Optional preferred PBS length to highlight; a full 8-17 nt sweep is always returned."
},
"replaceEnd": {
"type": "integer",
"description": "1-based inclusive end of the region being replaced/deleted. For a pure insertion, set replaceEnd = replaceStart - 1."
},
"newSequence": {
"type": "string",
"description": "New sequence (forward strand) to install in place of [replaceStart, replaceEnd]. Needs >= 2 bp."
},
"replaceStart": {
"type": "integer",
"description": "1-based inclusive start of the region being replaced/deleted."
},
"overlapLength": {
"type": "integer",
"default": 15,
"description": "Length (bp) of the shared overlap built into both pegRNAs' 3' flaps where they meet and anneal."
}
},
"additionalProperties": false
}No endpoints wrapped at confidence ≥ 0.70.
Parent server
com.seqbench/workbench
1/7 registries