primer_design

Parameters schema

{
  "type": "object",
  "required": [
    "template"
  ],
  "properties": {
    "mgMM": {
      "type": "number",
      "default": 1.5,
      "description": "Divalent cation [Mg2+] (mM)."
    },
    "naMM": {
      "type": "number",
      "default": 50,
      "description": "Monovalent cation [Na+]/[K+] (mM)."
    },
    "gcMax": {
      "type": "number",
      "default": 60
    },
    "gcMin": {
      "type": "number",
      "default": 40
    },
    "tmMax": {
      "type": "number",
      "default": 63
    },
    "tmMin": {
      "type": "number",
      "default": 57
    },
    "tmOpt": {
      "type": "number",
      "default": 60
    },
    "dntpMM": {
      "type": "number",
      "default": 0.2,
      "description": "Total [dNTP] (mM), chelates Mg2+."
    },
    "lenMax": {
      "type": "integer",
      "default": 25
    },
    "lenMin": {
      "type": "integer",
      "default": 18
    },
    "lenOpt": {
      "type": "integer",
      "default": 20
    },
    "oligoNM": {
      "type": "number",
      "default": 250,
      "description": "Total strand concentration (nM)."
    },
    "template": {
      "type": "string",
      "description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
    },
    "maxReturn": {
      "type": "integer",
      "default": 5,
      "description": "Number of best pairs to return."
    },
    "targetEnd": {
      "type": "integer",
      "description": "1-based inclusive end of the target region (optional)."
    },
    "tmMaxDiff": {
      "type": "number",
      "default": 3,
      "description": "Max Tm difference within a pair (°C)."
    },
    "ampliconMax": {
      "type": "integer",
      "default": 1000
    },
    "ampliconMin": {
      "type": "integer",
      "default": 100
    },
    "targetStart": {
      "type": "integer",
      "description": "1-based inclusive start of a region the product must span (optional)."
    }
  },
  "additionalProperties": false
}

No endpoints wrapped at confidence ≥ 0.50.

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