in_silico_pcr

Active

declared in 1.1.0

Predict PCR products for a template and a pair of primers (IUPAC-aware, allows mismatches, handles circular templates).

Parameters schema

{
  "type": "object",
  "required": [
    "template",
    "forwardPrimer",
    "reversePrimer"
  ],
  "properties": {
    "circular": {
      "type": "boolean",
      "default": false,
      "description": "Treat the template as circular (plasmid)."
    },
    "template": {
      "type": "string",
      "description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
    },
    "forwardPrimer": {
      "type": "string",
      "description": "Primer 1, 5'→3'."
    },
    "maxMismatches": {
      "type": "integer",
      "default": 0,
      "description": "Mismatches tolerated per primer."
    },
    "reversePrimer": {
      "type": "string",
      "description": "Primer 2, 5'→3' (order does not matter)."
    }
  },
  "additionalProperties": false
}

What this tool wraps· 0 endpoints

min confidence0.70 0.50

No endpoints wrapped at confidence ≥ 0.70.

Parent server

com.seqbench/workbench

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