fastq_trim
ActiveTool of com.seqbench/workbench
Trim FASTQ reads: an ungapped sliding-suffix adapter match (against the same named Illumina adapters as the QC report) followed by a BWA-style 3' quality trim (the same algorithm Cutadapt's own -q option reuses), then drops reads below a minimum length. Returns the trimmed FASTQ plus before/after read-count, mean-length and mean-quality stats.
Parameters schema
{
"type": "object",
"required": [
"input"
],
"properties": {
"input": {
"type": "string",
"description": "FASTQ text: records of an '@id' header, sequence, '+' separator and quality line (four lines each)."
},
"minLength": {
"type": "integer",
"default": 20,
"description": "Reads shorter than this after trimming are dropped."
},
"qualityOffset": {
"enum": [
33,
64
],
"type": "integer",
"default": 33,
"description": "FASTQ Phred ASCII offset (33 = Sanger/Illumina 1.8+, 64 = Illumina 1.3-1.7)."
},
"qualityThreshold": {
"type": "integer",
"default": 20,
"description": "3' quality-trim threshold (Phred score)."
}
},
"additionalProperties": false
}No endpoints wrapped at confidence ≥ 0.50.
Parent server
com.seqbench/workbench
1/7 registries