melting_temperature

Parameters schema

{
  "type": "object",
  "required": [
    "sequence"
  ],
  "properties": {
    "mgMM": {
      "type": "number",
      "default": 1.5,
      "description": "Divalent cation [Mg2+] (mM)."
    },
    "naMM": {
      "type": "number",
      "default": 50,
      "description": "Monovalent cation [Na+]/[K+] (mM)."
    },
    "dntpMM": {
      "type": "number",
      "default": 0.2,
      "description": "Total [dNTP] (mM), chelates Mg2+."
    },
    "oligoNM": {
      "type": "number",
      "default": 250,
      "description": "Total strand concentration (nM)."
    },
    "sequence": {
      "type": "string",
      "description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
    },
    "targetTm": {
      "type": "number",
      "description": "Optional target Tm (°C). When given, the response includes a pass/fail gate."
    },
    "tmTolerance": {
      "type": "number",
      "default": 2,
      "description": "Allowed +/- window (°C) around targetTm for the gate."
    }
  },
  "additionalProperties": false
}

No endpoints wrapped at confidence ≥ 0.50.

Parent server