primer_specificity
ActiveTool of com.seqbench/workbench
declared in 1.1.0
Self-hosted e-PCR-style screen for off-target amplicons predicted by a primer pair against a small set of curated reference genomes (currently: E. coli K-12 MG1655 — see genomesChecked for the exact list). This checks background/host-genome specificity, NOT whether the primers hit your intended target — pair it with in_silico_pcr against your own template for that. Batchable over candidate REVERSE primers against one fixed forward primer (screen many candidates against a shared partner) — not independent primer-pair batching, which this tool doesn't support.
Parameters schema
{
"type": "object",
"required": [
"forwardPrimer",
"reversePrimer"
],
"properties": {
"forwardPrimer": {
"type": "string",
"description": "Forward primer, 5'→3'."
},
"maxMismatches": {
"type": "integer",
"default": 1,
"description": "Mismatches tolerated per primer against a reference genome."
},
"reversePrimer": {
"type": "string",
"description": "Reverse primer, 5'→3'."
},
"maxProductLength": {
"type": "integer",
"default": 5000,
"description": "Ignore candidate off-target products longer than this (bp) — a search-window cap, not a biological claim."
}
},
"additionalProperties": false
}No endpoints wrapped at confidence ≥ 0.50.
Parent server
com.seqbench/workbench
1/7 registries