sequencing_readback_verify

Active

declared in 1.1.0

Align raw Sanger or NGS reads (FASTA or FASTQ) back onto a claimed reference sequence using minimap2, and report per-read mapping identity plus exact variant positions (substitutions/insertions/deletions), with a consensus view across reads and a corrected consensus sequence (the reference with every consensus-supported edit applied). Complements verify_construct/verify_assembly: those re-derive what a design SHOULD produce from its own stated inputs; this checks what a real sequencer actually read back.

Parameters schema

{
  "type": "object",
  "required": [
    "reference",
    "reads"
  ],
  "properties": {
    "reads": {
      "type": "string",
      "description": "Raw reads in FASTA or FASTQ format (auto-detected). Up to 2000 reads / 5,000,000 total bp per call."
    },
    "reference": {
      "type": "string",
      "description": "The claimed/expected reference sequence."
    },
    "minSupportingReads": {
      "type": "integer",
      "default": 2,
      "description": "Minimum number of reads agreeing on a variant position for it to count as a consensus (candidate real) variant rather than single-read noise."
    }
  },
  "additionalProperties": false
}

What this tool wraps· 0 endpoints

min confidence0.70 0.50

No endpoints wrapped at confidence ≥ 0.50.

Parent server

com.seqbench/workbench

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