crispr_offtarget_check

Active

declared in 1.1.0

Screen a guide's protospacer for off-target sites (protospacer match + valid PAM, both strands) against a small curated set of common lab reference genomes (see genomesChecked) — NOT a whole human/mouse genome search. Use this the same way primer_specificity is used: a useful sanity check within the covered organisms, not a clearance guarantee for a mammalian expression host.

Parameters schema

{
  "type": "object",
  "required": [
    "protospacer"
  ],
  "properties": {
    "nuclease": {
      "enum": [
        "spcas9",
        "spcas9ng",
        "sacas9",
        "cas12a"
      ],
      "type": "string",
      "default": "spcas9",
      "description": "Nuclease id — determines the PAM pattern/side required at each candidate site."
    },
    "protospacer": {
      "type": "string",
      "description": "The guide's protospacer sequence, 5'→3' (no PAM)."
    },
    "maxMismatches": {
      "type": "integer",
      "default": 2,
      "description": "Mismatches tolerated between the protospacer and a candidate genomic site."
    }
  },
  "additionalProperties": false
}

What this tool wraps· 0 endpoints

min confidence0.70 0.50

No endpoints wrapped at confidence ≥ 0.70.

Parent server

com.seqbench/workbench

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