sanger_vs_reference
ActiveTool of com.seqbench/workbench
declared in 1.1.0
Align a Sanger ABIF read to a reference and report identity plus every mismatch, insertion and deletion.
Parameters schema
{
"type": "object",
"required": [
"reference"
],
"properties": {
"read": {
"type": "string",
"description": "Sanger read as FASTA or raw text (alternative to uploading an ABIF trace). Also the per-record field for plate-batch runs via /api/v1/batch."
},
"fileName": {
"type": "string",
"description": "Optional original file name (echoed back)."
},
"reference": {
"type": "string",
"description": "Expected reference sequence (FASTA or raw)."
},
"fileBase64": {
"type": "string",
"description": "The binary ABIF (.ab1 / .abi) trace file, base64-encoded."
},
"minCoverage": {
"type": "number",
"default": 0.9,
"description": "Fraction of the reference the read must span before a PASS is meaningful; below this the verdict is 'ambiguous_low_coverage' regardless of identity. Lower it when the reference is intentionally just the region/junction being checked."
}
},
"additionalProperties": false
}No endpoints wrapped at confidence ≥ 0.50.
Parent server
com.seqbench/workbench
1/7 registries