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PRSM

sanger_vs_reference

Active

Tool of com.seqbench/workbench

declared in 1.1.0

Align a Sanger ABIF read to a reference and report identity plus every mismatch, insertion and deletion.

Parameters schema

{
  "type": "object",
  "required": [
    "reference"
  ],
  "properties": {
    "read": {
      "type": "string",
      "description": "Sanger read as FASTA or raw text (alternative to uploading an ABIF trace). Also the per-record field for plate-batch runs via /api/v1/batch."
    },
    "fileName": {
      "type": "string",
      "description": "Optional original file name (echoed back)."
    },
    "reference": {
      "type": "string",
      "description": "Expected reference sequence (FASTA or raw)."
    },
    "fileBase64": {
      "type": "string",
      "description": "The binary ABIF (.ab1 / .abi) trace file, base64-encoded."
    },
    "minCoverage": {
      "type": "number",
      "default": 0.9,
      "description": "Fraction of the reference the read must span before a PASS is meaningful; below this the verdict is 'ambiguous_low_coverage' regardless of identity. Lower it when the reference is intentionally just the region/junction being checked."
    }
  },
  "additionalProperties": false
}

What this tool wraps· 0 endpoints

min confidence0.700.50

No endpoints wrapped at confidence ≥ 0.50.

Parent server

com.seqbench/workbench

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sanger_vs_reference — com.seqbench/workbench — PRSM MCP