site_directed_mutagenesis

Parameters schema

{
  "type": "object",
  "required": [
    "template"
  ],
  "properties": {
    "mgMM": {
      "type": "number",
      "default": 1.5,
      "description": "Divalent cation [Mg2+] (mM)."
    },
    "naMM": {
      "type": "number",
      "default": 50,
      "description": "Monovalent cation [Na+]/[K+] (mM)."
    },
    "style": {
      "enum": [
        "quikchange",
        "q5"
      ],
      "type": "string",
      "default": "quikchange",
      "description": "Mutagenic primer style."
    },
    "dntpMM": {
      "type": "number",
      "default": 0.2,
      "description": "Total [dNTP] (mM), chelates Mg2+."
    },
    "newBase": {
      "enum": [
        "A",
        "C",
        "G",
        "T"
      ],
      "type": "string",
      "description": "Replacement base (editKind='nt')."
    },
    "oligoNM": {
      "type": "number",
      "default": 250,
      "description": "Total strand concentration (nM)."
    },
    "residue": {
      "type": "integer",
      "description": "1-based residue number to change (editKind='aa')."
    },
    "editKind": {
      "enum": [
        "nt",
        "aa"
      ],
      "type": "string",
      "default": "aa",
      "description": "Edit at the nucleotide or amino-acid level."
    },
    "organism": {
      "enum": [
        "ecoli",
        "human",
        "yeast",
        "cho",
        "pichia",
        "insect",
        "arabidopsis",
        "zebrafish"
      ],
      "type": "string",
      "default": "ecoli",
      "description": "Codon-usage table for choosing the new codon (editKind='aa')."
    },
    "position": {
      "type": "integer",
      "description": "1-based position to substitute (editKind='nt')."
    },
    "targetAa": {
      "type": "string",
      "description": "Target amino acid, one-letter code incl '*' (editKind='aa')."
    },
    "template": {
      "type": "string",
      "description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
    },
    "frameStart": {
      "type": "integer",
      "default": 1,
      "description": "1-based position of the first base of codon 1 (editKind='aa')."
    },
    "armTmTarget": {
      "type": "number",
      "default": 60,
      "description": "Target Tm (°C) for each template-binding arm."
    }
  },
  "additionalProperties": false
}

No endpoints wrapped at confidence ≥ 0.70.

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