crispr_grna_design
ActiveTool of com.seqbench/workbench
declared in 1.1.0
Find and score candidate guide RNAs (protospacer + PAM) in a target DNA for common nucleases (SpCas9, SpCas9-NG, SaCas9, Cas12a).
Parameters schema
{
"type": "object",
"required": [
"sequence"
],
"properties": {
"minScore": {
"type": "number",
"default": 0,
"description": "Only return guides with a heuristic score at least this high (0–100)."
},
"nuclease": {
"enum": [
"spcas9",
"spcas9ng",
"sacas9",
"cas12a"
],
"type": "string",
"default": "spcas9",
"description": "Nuclease id. Omit to just list the available nucleases (no scan is performed)."
},
"sequence": {
"type": "string",
"description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
},
"searchReverseStrand": {
"type": "boolean",
"default": true,
"description": "Also scan the reverse strand for guides."
}
},
"additionalProperties": false
}No endpoints wrapped at confidence ≥ 0.70.
Parent server
com.seqbench/workbench
1/7 registries