You're viewing a demo portfolio

Join the waitlist
PRSM

crispr_grna_design

Active

Tool of com.seqbench/workbench

declared in 1.1.0

Find and score candidate guide RNAs (protospacer + PAM) in a target DNA for common nucleases (SpCas9, SpCas9-NG, SaCas9, Cas12a).

Parameters schema

{
  "type": "object",
  "required": [
    "sequence"
  ],
  "properties": {
    "minScore": {
      "type": "number",
      "default": 0,
      "description": "Only return guides with a heuristic score at least this high (0–100)."
    },
    "nuclease": {
      "enum": [
        "spcas9",
        "spcas9ng",
        "sacas9",
        "cas12a"
      ],
      "type": "string",
      "default": "spcas9",
      "description": "Nuclease id. Omit to just list the available nucleases (no scan is performed)."
    },
    "sequence": {
      "type": "string",
      "description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
    },
    "searchReverseStrand": {
      "type": "boolean",
      "default": true,
      "description": "Also scan the reverse strand for guides."
    }
  },
  "additionalProperties": false
}

What this tool wraps· 0 endpoints

min confidence0.700.50

No endpoints wrapped at confidence ≥ 0.50.

Parent server

com.seqbench/workbench

1/7 registries
View full server →