base_editing_design

Active

declared in 1.1.0

Design cytosine (CBE, C→T) or adenine (ABE, A→G) base-editing gRNAs for an SpCas9 target: for each NGG gRNA it reports every editable base inside the editor's activity window, flags bystander edits (more than one editable base in the window), and — with a CDS reading frame — classifies each edit's amino-acid consequence (silent / missense / nonsense / stop-loss). Bystander-free guides are ranked first. Handles both strands (a C→T on the protospacer of a reverse-strand guide is reported as the forward-strand G→A).

Parameters schema

{
  "type": "object",
  "required": [
    "target"
  ],
  "properties": {
    "editor": {
      "enum": [
        "be3",
        "be4max",
        "abe7.10",
        "abe8e"
      ],
      "type": "string",
      "default": "be4max",
      "description": "Base editor: be3/be4max (CBE, C→T) or abe7.10/abe8e (ABE, A→G)."
    },
    "target": {
      "type": "string",
      "description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
    },
    "frameStart": {
      "type": "integer",
      "description": "Optional 1-based CDS reading-frame start, to classify each edit's amino-acid consequence."
    },
    "targetPosition": {
      "type": "integer",
      "description": "Optional 1-based forward-strand position of the base you intend to edit; only guides whose window covers it are returned."
    }
  },
  "additionalProperties": false
}

What this tool wraps· 0 endpoints

min confidence0.70 0.50

No endpoints wrapped at confidence ≥ 0.70.

Parent server

com.seqbench/workbench

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