base_editing_design
ActiveTool of com.seqbench/workbench
declared in 1.1.0
Design cytosine (CBE, C→T) or adenine (ABE, A→G) base-editing gRNAs for an SpCas9 target: for each NGG gRNA it reports every editable base inside the editor's activity window, flags bystander edits (more than one editable base in the window), and — with a CDS reading frame — classifies each edit's amino-acid consequence (silent / missense / nonsense / stop-loss). Bystander-free guides are ranked first. Handles both strands (a C→T on the protospacer of a reverse-strand guide is reported as the forward-strand G→A).
Parameters schema
{
"type": "object",
"required": [
"target"
],
"properties": {
"editor": {
"enum": [
"be3",
"be4max",
"abe7.10",
"abe8e"
],
"type": "string",
"default": "be4max",
"description": "Base editor: be3/be4max (CBE, C→T) or abe7.10/abe8e (ABE, A→G)."
},
"target": {
"type": "string",
"description": "Nucleotide sequence (raw or FASTA; IUPAC accepted)."
},
"frameStart": {
"type": "integer",
"description": "Optional 1-based CDS reading-frame start, to classify each edit's amino-acid consequence."
},
"targetPosition": {
"type": "integer",
"description": "Optional 1-based forward-strand position of the base you intend to edit; only guides whose window covers it are returned."
}
},
"additionalProperties": false
}No endpoints wrapped at confidence ≥ 0.50.
Parent server
com.seqbench/workbench
1/7 registries